mouse anti-cc1 calbiochem Search Results


anti adenomatous polyposis coli  (R&D Systems)
96
R&D Systems anti adenomatous polyposis coli
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Anti Adenomatous Polyposis Coli, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+calbiochem/Mouse+PDGF+R+alpha+Antibody/pmc10644703-90-15-26
Average 96 stars, based on 1 article reviews
anti adenomatous polyposis coli - by Bioz Stars, 2026-10
96/100 stars
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mouse pdgf r alpha antibody  (Bio-Techne corporation)
98
Bio-Techne corporation mouse pdgf r alpha antibody
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Mouse Pdgf R Alpha Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+calbiochem/Mouse+PDGF+R+alpha+Antibody/custom%40af1062%4031753579
Average 98 stars, based on 1 article reviews
mouse pdgf r alpha antibody - by Bioz Stars, 2026-10
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mouse anti cc1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mouse anti cc1
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Mouse Anti Cc1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+calbiochem/CEACAM1+Antibody/pmc05728697-439-22-24
Average 93 stars, based on 1 article reviews
mouse anti cc1 - by Bioz Stars, 2026-10
93/100 stars
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human/mouse carbonic anhydrase ii/ca2 antibody  (Bio-Techne corporation)
93
Bio-Techne corporation human/mouse carbonic anhydrase ii/ca2 antibody
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Human/Mouse Carbonic Anhydrase Ii/Ca2 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+calbiochem/Human%2FMouse+Carbonic+Anhydrase+II%2FCA2+Antibody/bio-techne+corporation___mab2184
Average 93 stars, based on 1 article reviews
human/mouse carbonic anhydrase ii/ca2 antibody - by Bioz Stars, 2026-10
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mouse anti mbp  (Covance)
86
Covance mouse anti mbp
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Mouse Anti Mbp, supplied by Covance, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+calbiochem/anti+mbp/10__1523_slash_jneurosci__2113___16__2017-71-16-20
Average 86 stars, based on 1 article reviews
mouse anti mbp - by Bioz Stars, 2026-10
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aif-1/iba1 antibody  (Bio-Techne corporation)
99
Bio-Techne corporation aif-1/iba1 antibody
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Aif 1/Iba1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+calbiochem/AIF-1%2FIba1+Antibody/custom%40nb100-1028%4010%2E1101%2F796474
Average 99 stars, based on 1 article reviews
aif-1/iba1 antibody - by Bioz Stars, 2026-10
99/100 stars
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goat anti-pdgfrα  (GeneTex)
90
GeneTex goat anti-pdgfrα
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Goat Anti Pdgfrα, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+calbiochem/goat+anti+pdgfr%CE%B1/pmc06916379-59-6-8
Average 90 stars, based on 1 article reviews
goat anti-pdgfrα - by Bioz Stars, 2026-10
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goat anti mouse secondary antibody  (Vector Laboratories)
96
Vector Laboratories goat anti mouse secondary antibody
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Goat Anti Mouse Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-cc1+calbiochem/Unconjugated+Goat+Anti-Mouse+IgG+Antibody/pmc03178147-101-15-25
Average 96 stars, based on 1 article reviews
goat anti mouse secondary antibody - by Bioz Stars, 2026-10
96/100 stars
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anti gfap  (Neuromics)
93
Neuromics anti gfap
Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Anti Gfap, supplied by Neuromics, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Anti Gfp, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti-pdgfrα  (Becton Dickinson)
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Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Rat Anti Pdgfrα, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti-cd31  (Becton Dickinson)
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Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC <t>(CC1),</t> OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.
Rat Anti Cd31, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC (CC1), OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.

Journal: Frontiers in Cellular Neuroscience

Article Title: Activity-dependent oligodendrocyte calcium dynamics and their changes in Alzheimer’s disease

doi: 10.3389/fncel.2023.1154196

Figure Lengend Snippet: Neuronal activity-dependent Ca 2+ activities in oligodendrocytes (OCs). (A,B) Representative images (A) and quantification (B) of GCaMP expression in the motor cortex of PLP-GCaMP mice and co-localization with markers for OC + oligodendrocyte precursor cell (OPC) (Olig2), OC (CC1), OPC (PDGFRα, NG2), neuron (NeuN), astrocyte (S100β) (Olig2 [83.41 ± 3.899%], CC1 [72.65 ± 3.464%], PDGFRα+ [9.979 ± 0.9322%], NG2 [6.736 ± 0.2401%], NeuN [1.481 ± 0.7246%], and S100β [8.156 ± 0.5504%]). Scale bar, 30 μm. (C,D) Representative images of MBP immunostaining in the motor cortex of PLP-GCaMP6 mice. Scale bars: in (C) , 100 μm; in (D) , 30 μm. (E) Quantitative analysis of the co-localization areas of MBP and GCaMP’ + ve processes based on immunostaining data. The proportion of MBP’ + ve processes in the processes of GCaMP’ + ve cells was about 15%. (F) Experimental protocol of Ca 2+ imaging in OCs. The first craniotomy was performed one week before Pre-imaging (Pre) using two-photon microscopy. After Pre-imaging, a second craniotomy was performed and TTX was applied for 30 min, followed by a second imaging (TTX) of the same cells. (G,H) Representative image of GCaMP’ + ve cells of the motor cortex in PLP-GCaMP6 mice before and after TTX application. Red spots indicate the Ca 2+ activated areas (spot). Scale bar = 30 μm. Representative Ca 2+ traces from spots of typical GCaMP’ + ve cells are shown. (I) Changes in Ca 2+ spots, Ca 2+ events and total area under the curve (AUC) of GCaMP’ + ve cells between before and after TTX application. The number of Ca 2+ spots and Ca 2+ events and total AUC were significantly decreased after TTX application. Pre: n = 6 mice, 13 imaging fields (cells); TTX: n = 6 mice, 13 imaging fields (cells). * p < 0.05, ** p < 0.01, Mann–Whitney U test. Data are presented as mean ± standard error of mean. For detailed data, check the source data file. (J) Changes in AUC, Amplitude, and Latency of GCaMP’ + ve cells between before and after TTX application. AUC was not significantly changed, Amplitude was significantly decreased, and Latency was significantly increased after TTX application. Pre: n = 6 mice, 426 events; TTX: n = 6 mice, 189 events. N.S., not significant, * p < 0.05, *** p < 0.001, Mann–Whitney U test. Violin plots show median (black line) and distribution of the data. For detailed data, check the source data file. (K) Proportions of AUC and Latency were not significantly changed between before and after TTX application. The proportion of lower Amplitude was significantly increased after TTX application. N.S., not significant, ** p < 0.01, Kolmogorov–Smirnov test. For detailed data, check the source data file.

Article Snippet: The primary antibodies used in this study were as follows: anti-Olig2 (rabbit; Millipore, AB9610; 1:1000), anti-adenomatous polyposis coli (clone CC1) (mouse; Calbiochem, OP80; 1:500), anti-PDGFRα (goat; R&D systems, AF1062; 1:200), anti-NG2 (rabbit; Millipore, AB5320; 1:500), anti-NeuN (mouse; Millipore, MAB377; 1:500), anti-S100β (rabbit; Abcam, ab52642; 1:1000), anti-MBP (mouse; BioLegend, clone SMI 99; 1:100), and anti-GFP (chicken; Novus biologicals, NB100-1614; 1:2000).

Techniques: Activity Assay, Expressing, Immunostaining, Imaging, Microscopy, MANN-WHITNEY